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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Circ Cardiovasc Genet. 2012 Oct 24;5(6):621–629. doi: 10.1161/CIRCGENETICS.112.964064

Figure 5.

Figure 5

TGFβ1 and TGFβ2 signaling in dermal fibroblasts. A–B. Cultured fibroblasts were isolated from normal patient with wt-TGFbRII and ANV I-1 patient with H56N-TGFbRIIb mutation. Representative Western blot analyses shown for pSMAD2 protein expression in wt-TGFbRII or H56N-TGFbRIIb fibroblasts stimulated with either TGFb1 (A) or TGFb2 (B) for indicated time points. Corresponding b-actin blots shown. (A) H56N-TGFbRIIb dermal fibroblasts exhibit delayed TGFβ1 signaling compared to normal dermal fibroblasts. (B) H56N-TGFbRIIb dermal fibroblasts exhibit decreased TGFβ2 signaling compared to normal dermal fibroblasts.