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. Author manuscript; available in PMC: 2013 Jan 17.
Published in final edited form as: Urology. 2010 May 15;76(4):1018.e8–1018.13. doi: 10.1016/j.urology.2010.02.055

Figure 3.

Figure 3

Effects of TGF-β1 and estrogen on urethral elastin mRNA expression. Urethral cells were isolated from female rats, treated with TGF-β1 and/or estrogen, and analyzed by real-time PCR. A. Dosage response of elastin mRNA expression to TGF-β1. “Relative expression” is the ratio of elastin versus GAPDH expression. B. Elastin mRNA expression in urethral cells treated with 1 ng/ml TGF-β1 (TGF), 10 nM estrogen (E2), 10 nM estrogen for 1 h and then 1 ng/ml TGF-β1 for 16 h (E2/TGF), or 1 ng/ ml TGF-β1 for 1 h and then 10 nM estrogen for 16 h (TGF/E2). “Relative expression” is the ratio of elastin versus GAPDH expression. * indicates significant difference (p<0.05) compared to TGF-β1 alone. The data are the average of 3 independent experiments.