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Sequential click approach for dynamically tuning extracellular microenvironments: (a) step-growth network formation via SPAAC that enables thiol–ene photopatterning without altering the original network structure by employing 4-arm PEG tetra azide and dicyclooctyne MMP cleavable (↓ shows cleavage site) peptide; (b) spatial thiol–ene photopatterning of a first biochemical unit; (c) patterning of a second biochemical unit at a different time point and location.
