A–C) A total of 4×106 MMC-hDSG2 cells were injected into the mammary fat pad of hDSG2 transgenic mice. When tumors reached a volume of 100 mm3, JO-1 (2 mg/kg) was injected intravenously. Mice were sacrificed 6 hours later, blood was removed from the circulation, and organs were harvested. A and B) Sections of MMC-hDSG2 tumors and intestine stained for JO-1 (red) and hDSG2 (green). C and D). JO-1 biodistribution: JO-1 (2 mg/kg) was injected intravenously. Six hours later, blood was flushed from the circulation and tissue lysates were analyzed for JO-1 by ELISA. n=3. ***P<0.001, n.s. non-signficant. E and F) Tumor (E) and intestine (F) sections from MMC-hDSG2 tumor bearing hDSG2 transgenic mice were stained for the adherens junction marker claudin 7 (red) and hDSG2 (green). Representative sections are shown. The scale bars are 20 μm.