Table 1.
Oligonucleotides used for plasmid construction
| Plasmid | contains | Oligonucleotide | Sequence (5'-3')* |
|---|---|---|---|
| pMA319 |
UL51 |
MOL269 |
ATGTCCTGGGCTAAGCAGCG |
| MOL270 |
TTATTTACCCGGCGCCGACT |
||
| pMA48 |
UL56 (5’) |
MOL60 |
CCATGGAGATGAATTTGTTAC |
| MOL64 |
CTCCTCGAGAATATGCTTGAT |
||
| pMA49 |
UL56 (3’) |
MOL59 |
ACAGGTTAACGCAGACTACCA |
| MOL65 |
ATATTCTCGAGGAGATTCGAC |
||
| pUL89 |
UL89 cDNA |
MOL53 |
CTGCTGCGTGCCTAGCTGAC |
| MOL54 |
CATCATGTTGCGCGGAGACT |
||
| pMA323 |
FLAG-UL51 |
UL51-FLAG-H3F |
GACCTAAGCTTTCCTGGGCTAAGCAGCGGGTG |
| UL51-FLAG-ER1R |
TACGATGAATTCTTATTTACCCGGCGCCGACTC |
||
| pMA326 |
FLAG-UL51 |
UL51-FLAG-BamHI |
CGGGATCCATGGACTACAAGGACGACGA |
| UL51-FLAG-ER1R |
TACGATGAATTCTTATTTACCCGGCGCCGACTC |
||
| pMA325 |
UL56 |
UL56-V5-F |
GAGATGAATTTGTTACAGAAACTA |
| UL56-V5-R |
TTAACGCAGACTACCAGGCAC |
||
| pMA324 | MYC-UL89 | linkA |
GATCCACCATGGAACAAAAACTCATCTCAGAAGAGGATCTGG |
| linkB |
AATTCCAGATCCTCTTCTGAGATGAGTTTTTGTTCCATGGTG |
||
| UL89-NMyc-ER1F |
CATGATGAATTCTTGCGCGGAGACTCGGCC |
||
| UL89-NMyc-XB1R | TACATGTCTAGACTAGCTGACCCTGAAACG |
*restriction sites used for cloning are underlined.