Table 1.
Oligonucleotide primers used in the study
| PCR reaction / Primer (Polarity) | Sequence (5′to 3′) | Positiona | PCR product |
|---|---|---|---|
|
Primary |
|
|
508 bp |
| HPIV2-cDNA (+) |
AACAATCTGCTGCAGCATTT |
7437-7456 |
|
| HPIV2-rev (−) |
ATGTCAGACAATGGGCAAAT |
7925-7944 |
|
|
Nested |
|
|
204 bp |
| HPIV2-nF(+) |
CCATTTACCTAAGTGATGGAAT |
7479-7500 |
|
| HPIV2-nR(−) |
GCCCTGTTGTATTTGGAAGAGA |
7661-7682 |
|
|
Sequencing |
|
|
|
| HPIV2-HN-F1(+) |
CGAACCCTTAAGGTGTCGTAACGTC |
6648-6672 |
886 bp |
| HPIV2-HN-R1 (−) |
GGTATAGCAGTGACTGAACAGC |
7512-7533 |
|
| HPIV2-HN-F2(+) |
GACCCATTGGTGTTACACTCACAATG |
7348-7373 |
840 bp |
| HPIV2-HN-R2(−) |
GTTGCATTGCATGGCATGACTC |
8166-8187 |
|
| HPIV2-HN-F3(+) |
GGTACCGTCCTATCAAGTTCC |
8137-8157 |
426 bp |
| HPIV2-HN-R3 (−) | GAATCTGGAGTCTTCATTCAATAG | 8539-8562 |
a Nucleotide positions based on the complete genome sequence of HPIV-2 strain Greer; GenBank accession number AF533012.