Figure 2.

The assessment of the expression and purification of anophelines salivary proteins. Recombinant ortholog forms of SG6 and 5′nucleotidases from An. gambiae and An. funestus expressed in Spodoptera frugiperda (Sf9) cells and purified by affinity and gel filtration chromatography were separated on a 15% SDS-PAGE and post-stained with Imperial™ Protein Stain (Thermo Scientific). Five micrograms of each collected fraction were loaded per well. The protein name and the Anopheles species corresponding to each well are indicated at the top of the gel. The Roman numeral numbers on the right side of the gel correspond to the band numbers excised for identification by mass spectrometry. Band identity is listed in Table 2. Standard molecular weights are indicated on the left side. MW: molecular weight. kDa: kilodalton.