Table 3.

Sequence comparison of the P1 region of A24 Cruzeiro and A-SIRs

Gene	A24 Cruzeiro WT			A-SIR #15 and #23 (Class I)			A-SIR #42 and #45 (Class II)
	Nucleotidea	Codonb	Amino acidc	Nucleotidea	Codonb	Amino acidc	Nucleotidea	Codonb	Amino acidc
VP2	C50	cgG	----	----	----	----	G5045	cgA	Silent
VP1	G6	acG	----	C6	acC	Silent	C6	acC	Silent
VP1	G283	Gaa	E95	----	----	----	A28342	Aaa	K95
VP1	C287	tCa	S96	----	----	----	T28742	tTa	L96
VP1	G434	gGc	G145	A434	gAc	D145	----	----	----
VP1	T449	cTc	L150	----	----	----	C44942	cCc42	P15042
							G44945	cGc45	R15045
VP1	T461	gTc	V154	C46123	gCc	A15423	----	----	----

^a Nucleotide differences between viruses followed by number indicating its position within the gene coding sequence.

^b Lowercase letters indicate bases shared by the 2 viruses and capital letters indicate bases differing between the viruses.

^c One-letter code of the encoded amino acid residues followed by number indicating residue position in the polypeptide.

P1 region of A24 Cruzeiro (Accession # AY596768) was compared against: Class I (#15 and #23) and Class II (#42 and #45) A-SIRs. Nucleotide changes relative to WT were indicated and codon positions. Silent mutations and amino acid substitutions are indicated. A-SIR mutant numerical designations were superscripted.