Table 1.
Intra and inter-assay variations in PPRNT
| Virus Input | Intra-assay variation | Inter-assay variation | |||||
|---|---|---|---|---|---|---|---|
| |
Ratio between duplicate test in same serum |
*Six sera with known FFRNT |
|||||
| |
<4 |
4-7 |
7-10 |
Total |
32 (n=2) |
128 (n=2) |
512 (n=2) |
| |
n (%) |
n (%) |
n (%) |
n |
**GMT(CV) |
GMT(CV) |
GMT(CV) |
| 50 PPFU/well |
20 (28.98) |
33 (47.85) |
16 (23.18) |
69 |
36.76 (4.63) |
111.4 (7.70) |
445.70 (10.81) |
| 27.86 (9.19) | 147.0 (4.79) | 588.10 (7.53) | |||||
*Neutralizing anti-CCHF antibodies titers were directly assigned to the highest dilution with > 50% reduction.
**GMT; geometric mean titer, CV; coefficient of variance = (S.D./mean) × 100%, which was calculated with logarithmic transformation.