Table 1.
Primers used for genotyping and sequencing
| Gene ID | Chr. | Primer sequences (Forward/reverse) | Size | Thermal Conditions |
|---|---|---|---|---|
|
FOXP3 |
X |
5′-AGGCTGAGGGCCTCAGAAGCATC-3′* |
429 bp |
94°C(5mn), [94°C(15S), 61°C(1mn) 72°C(1mn)]x35, 72°C(10mn) |
| 5′-CTCCAGGCCTCAGTTTCCCTATAG-3′ | ||||
|
IL10RA |
11 |
5′-GGGAAGGAAAGGGAGGGGTGGC-3′* |
241 bp |
94°C(2mn), [94°C(30S) 61°C(30S),72°C(30S)]x35, 72°C(2mn) |
| 5′-GCGCGCCTCCAGCTACCCTTG-3′ | ||||
|
IL10RB |
21 |
5′-AAGGATGAGGCTGTGAGGAGG-3′* |
401 bp |
94°C(2mn), [94°C(30S) 61°C(30S),72°C(1mn)]x35, 72°C(2mn) |
| 5′-CAAGCAGAGGGAAGTGAATGCG-3′ | ||||
|
STAT6 |
12 |
5′-GGCGTGTCTCAGTGTTTACCCC-3′* |
530 bp |
94°C(2mn), [94°C(30S) 61°C(30S),72°C(1mn)]x30, 72°C(2mn) |
| 5′-GTCCCCCTCAAAAGCATCAGC-3′ | ||||
| TNFRSF18 | 1 | 5′-TCCATGGTTGAGGCTCTCTGG-3′* |
293 bp | 94°C(2mn), [94°C(30S) 61°C(30S),72°C(1mn)]x35, 72°C(2mn) |
| 5′-GTGTGAGGAGGGGGTGTAGAC-3′ |
Chr: Chromosome, *: Sequencing primers, bp: Base pairs, mn: minutes, S: seconds.