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α-HL was treated with 5 mM deoxycholate in the presence or absence of ORO. Following sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) analysis, the proteins were detected by silver staining. Lane 1, WT-HL; lane 2, T12A-HL; lane 3, I14A-HL; lane 4, WT-HL plus 8 µg/ml of ORO; lane 5, I14A-HL plus 8 µg/ml of ORO; lane 6, T12A-HL plus 8 µg/ml of ORO.
