Table 2. Values of the binding free energy and the number of binding sites (n) of α-HL-ORO systems based on the fluorescence-quenching method.
| WT | T12A | I14A | |
| ΔGbind (kcal/mol) | 8.4±0.2 | 6.3±0.1 | 6.9±0.1 |
| n | 1.0719 | 0.9945 | 0.9751 |
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