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. 2013 Jan 17;7(1):e2000. doi: 10.1371/journal.pntd.0002000

Figure 1. Anticipated reportable range and linearity of qPCR method.

Figure 1

Multiplex TaqMan qPCR strategy was carried out with spiked GEB samples containing parasite stocks belonging to TcI and TcVI in ten concentrations spanning 106 to 0.625 par. eq./10 mL, tested in triplicate. Assigned values were plotted on the x axis versus measured values (converted to log10) on the y axis using SigmaPlot 10.0 for Windows (SPSS, Chicago, IL). Linear regression analysis rendered the equation y = 1.013x+0.058 (R2 = 0.992) for TcI, and y = 1.001x+0.005 (R2 = 0.998) for TcVI.