Table 1. Sequences and concentrations of primers and probes used for the Multiplex Taqman qPCR assay.

Target	Oligonucleotide	Sequence	Final Concentration (µM)
T. cruzi satellite DNA	Cruzi 1	5′-ASTCGGCTGATCGTTTTCGA-3	0.75
	Cruzi 2	5′ -AATTCCTCCAAGCAGCGGATA-3	0.75
	Cruzi 3	5′ -Fam-CACACACTGGACACCAA-NFQ-MGB-3′	0.05
IAC	IAC Fw	5′ -ACCGTCATGGAACAGCACGTA-3′	0.1
	IAC Rv	5′ -CTCCCGCAACAAACCCTATAAAT-3′	0.1
	IAC Tq	5′ -VIC-AGCATCTGTTCTTGAAGGT-NFQ-MGB-3′	0.05

IAC: Internal amplification control. S: C/G.