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. 2013 Jan 17;7(1):e2017. doi: 10.1371/journal.pntd.0002017

Figure 4. Specificity of the mPCR approach in contaminated samples.

Figure 4

To mimic host derived contaminations of the template DNA, 5 ng of E. granulosus s.s. (G1) DNA was mixed in different ratios (1∶1 lane 1, 1∶2 lane 2, 1∶5 lane 3, 1∶10 lane 4, 1∶20 lane 5, 1∶30 lane 6, 1∶ 40 lane 7, 1∶ 50 lane 8, 1∶100 lane 9 and 1∶ 200 lane 10) with (A) DNA extracted from feces of a helminth-infection free dog and (B) calf thymus DNA. The background smear increased by applying more foreign DNA, but the genotype was still detectable, even when 1 µg total DNA was used as template (lane 9). M: 100-bp DNA ladder (Promega).