Table 1. Characteristics of oligonucleotides used for Echinococcus granulosus complex multiplex PCR.

Primer name	Conc. in mPCR	Product size (bp)	Specificity	Sequence 5′–3′a	Primer lenght (bp)	Gene marker	Acc No (NCBI)	Primer position
Echi Rpb2 F	1 µM	1232	All E. species	TTGACCAAAGAAATCAGAC	19	rpb2	FN566850.1	55–74
Echi Rpb2 R	1 µM	1232	All E. species	CGCAAATACTCCATGG	16	rpb2	FN566850.1	1287–1271
E.g complex F	0.15 µM	110	E. granulosus complex	TGGTCGTCTTAATCATTTG	19	cox2	AF297617.1	10686–10705
E.g complex R	0.15 µM	110	E. granulosus complex	CCACAACAATAGGCATAA	19	cox2	AF297617.1	10796–10777
E.g ss cal F	2 µM	1001	E. granulosus s.s. (G1/G/G3)	CAATTTACGGTAAAGCAT	18	cal	U834931.1	151–169
E.g ss cal R	2 µM	1001	E. granulosus s.s. (G1/G/G3)	CCTCATCTCCACTCTCT	17	cal	U834931.1	1152–1135
E.g ss Ef1a F	1 µM	706	E. granulosus s.s. (G1/G/G3)	TCCTAACATGCCTTGGTAT	19	ef1a	FN568380.1	594–613
E.g ss Ef1a R	1 µM	706	E. granulosus s.s. (G1/G/G3)	GTTACAGCCTTGATCACG	18	ef1a	FN568380.1	1300–1282
E.eq cal F	2 µM	426	E. equinus (G4)	GCTTATTTAGGATCCCA	17	cal	EU834936.1	566–583
E.eq cal R	2 µM	426	E. equinus (G4)	TCGTTTTTGCCAGTG	15	cal	EU834936.1	992–977
E.eq coxI F	0.2 µM	124	E. equinus (G4)	GTTGGgTTgGATGTT	15	cox1	M84664.1	143–158
E.eq coxI R	0.2 µM	124	E. equinus (G4)	CAAAACaGGATCACTCTT	18	cox1	M84664.1	277–259
E.ortp ATP6 F	0.05 µM	1041	E. ortleppi (G5)	GTGTCGTgTgTTTAgTGAG	19	atp-6	AF235846.1	6057–6076
E.ortp ATP6 R	0.05 µM	1041	E. ortleppi (G5)	GCACtGATAcaGGtGTtAtT	20	atp-6	AF235846.1	7098–7078
E.ortp CoxI F	0.2 µM	250	E. ortleppi (G5)	GGTTtTATGGGTTGTTA	17	cox1	AF235846.1	9978–9995
E.ortp CoxI R	0.2 µM	250	E. ortleppi (G5)	ACACCaCCAAACGTG	15	cox1	AF235846.1	10228–10213
E.cnd G6/G7 pold F	1 µM	617	E. canadensis (G6/G7)	GGCCTTCATCTCCATAATA	20	pold	FN568364.1	325–345
E.cnd G6/G7 pold R	1 µM	617	E. canadensis (G6/G7)	ATGAAGAGTTTGAAACTAAAG	21	pold	FN568364.1	942–921
E.cnd G6/G7 NDI F	0.3 µM	339	E. canadensis (G6/G7)	cTGCAGAGGTTTGCC	15	nad1	AB208063.1	7635–7650
E.cnd G6/G7 NDI R	0.3 µM	339	E. canadensis (G6/G7)	cACAACaGCAtAAAGCG	17	nad1	AB235847.1	7974–7957
E.cnd G8/G10 Elp F	1.5 µM	283	E. canadensis (G8/G10)	CCTAGTCTTCCCATGATA	18	elp1	U834894.1	450–468
E.cnd G8/G10 Elp R	1.5 µM	283	E. canadensis (G8/G10)	ACAGAAGGCATATCCA	16	elp1	U834894.1	733–717

^a) Strict specific bases in each primer are written in bold. Tiny characters mark additional polymorphic sites (but not strict).