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. 2013 Jan 17;8(1):e54172. doi: 10.1371/journal.pone.0054172

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© 2013 Schaefer et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A) Oscillating gray tone of subtracted bright field images (red) in comparison to the total fluorescence intensity of the corresponding TIRF images (green) and the impedance signal (black) as a function of time. The procedure allows to correlate the two independent experiments. B) Scheme of the two proposed states that amebas assume during TIRF microscopy explaining low fluorescence intensity (left) and high intensity (right). The minimal cell-substrate distance is not significantly undercut, only the overall contact zone increases leading to larger fluorescence intensity and impedance.