Figure 6. Clonogenic survival ability of cisplatin resistant cells is increased with increasing doses of cisplatin.

A549, SKMES-1, MOR and H460 PT and CisR cells were seeded in 6-well plates using optimised seeding densities. Following treatment with cisplatin for 72 h, media was removed and cells were allowed to recover for between 9–14 days after which time surviving colonies were stained using crystal violet stain and counted. The survival ability of CisR cells was significantly increased at various concentrations of cisplatin between cell lines relative to their parental counterparts, based on the number of colonies on plate following incubation with cisplatin. Data are expressed as Mean ± SEM from three independent experiments (n = 3) (^$p<0.05, ^#p<0.01, ^*p<0.001).