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. 2013 Jan 17;8(1):e51436. doi: 10.1371/journal.pone.0051436

Figure 4. MBD1 is a functional target of miR-195.

Figure 4

(A) The Mbd1-3′-UTR–dependent expression of Renilla luciferase reporter gene (R-Luc) was suppressed by miR-195 (n = 5, p<0.01) and enhanced by the inhibitor of miR-195 (anti-195) in aNSCs (n = 5, p = 0.087) compared with corresponding controls. (B) The mutation of the miR-195 target site in the Mbd1 3′ UTR abolished the repression by miR-195 and the enhancement by anti-195, meaning miR-195–mediated suppression of luciferase-Mbd1-3′-UTR was dependent on miR-195 target (n = 6, p>0.05). (C, D) Representative Western blot images (top panels) and quantification of independent Western blots (n = 4) showed that overexpression of miR-195 in WT aNSCs led to reduced endogenous MBD1 protein expression (C), whereas inhibition of miR-195 by anti-195 led to enhanced MBD1 protein expression in Mbd1 KO aNSCs (D). Data are presented as mean ± SEM; statistics were done using Student’s t-test*, p<0.05, **, p<0.01.