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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Mol Microbiol. 2012 Oct 5;86(4):988–1006. doi: 10.1111/mmi.12036

Figure 2.

Figure 2

The FhaB prodomain affects the surface-accessibility of MCD residues. (A) Schematic of B. bronchiseptica FhaB cysteine-substitution proteins. Single cysteine residues were substituted into wildtype (WT) and prodomain-truncated (ΔPD) proteins. (B) Cysteine-accessibility immunoblot of FHA recovered under native conditions from culture supernatants. The Western blots were immuno-stained with an anti-CRD antibody and displayed four isoforms of mature, released FHA. Native FHA was incubated with DyLight 680 Maleimide prior to SDS-PAGE.