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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Mol Microbiol. 2012 Oct 5;86(4):988–1006. doi: 10.1111/mmi.12036

Figure 3.

Figure 3

The C-terminus of the prodomain resides intracellularly during FHA maturation. (A) Schematic of B. bronchiseptica HA-tagged FhaB protein used in proteinase-K digestion experiments. The site of the HA tag is noted. (B) Left, anti-MCD and anti-HA immunoblot of proteinase-K treated whole-cells, lysed to evaluate cellular protein content. PMSF was added prior to proteinase-K incubation as indicated. The panel on the right is an analysis of the immunoblot on left, with the intensity of the 700 nm (anti-HA) channel increased for visualization of proteinase-K dependent intracellular prodomain. Bottom, anti-BvgS immunoblot of whole-cell lysates.