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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Mol Microbiol. 2012 Oct 5;86(4):988–1006. doi: 10.1111/mmi.12036

Figure 5.

Figure 5

The prodomain C-terminus of full-length FhaB remains intracellular. (A) Schematic of proteins used in dot blot experiments. (B) At the top, anti-MCD and anti-HA immuno-dot-blot of HAPRR and HA-ΔCS strains. Normalized amounts of whole cells and boiled lysates were applied. Below, illustrations of immuno-stained samples. The rounded gray rectangle represents the B. bronchiseptica outer membrane, with a solid edge representing the outer membrane of an intact cell and a dashed edge representing an outer membrane disrupted by boiling. MCD staining is represented by green-circled antibodies and HA staining is represented by red-circled antibodies.