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The prodomain of the HA-ΔMCT strain is mostly intracellularly-localized while that of the HA-ΔPNT strain is extracellularly-localized. (A) Schematic of proteins used in dot blot experiments. (B) At the top, anti-MCD and anti-HA immuno-dot-blot of HAPRR, HA-ΔMCT and HA-ΔPNT strains. Normalized amounts of whole cells and boiled lysates were applied. Below, illustrations of immuno-stained samples. Layout of these illustrations is as described in Figure 5B.
