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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: BJU Int. 2012 Jun 6;111(1):159–170. doi: 10.1111/j.1464-410X.2012.11278.x

FIG. 1.

FIG. 1

Top: characterization of the pelvic ganglia neurones that innervate the penis. Sections of sham-operated pelvic ganglia were immunostained with antibodies against nitric oxide synthase (nNOS) and antibodies against tyrosine hydroxylase (TH) and double co-localized with Fluorogold (FG)-labelled neurones. FG was injected in the penile corpora cavernosa 7 days before surgery and animal treatment. Blue: FG; red: nNOS; green: TH. Magnification × 400. Bottom: TH expression is not affected by bilateral cavernosal nerve resection (BCNR) and BCNR+sildenafil (BCNR+SIL). Sections of the pelvic ganglia from sham-operated (SHAM), BCNR and sildenafil animals 7 days after treatment were immunostained with anti-TH antibody and by biotin secondary antibody followed by streptavidin-fluorescein isothiocyanate. Green: TH positive neurones; yellow: FG positive neurones. Magnification × 100. Scale bar = 50μm.