Skip to main content
. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: BJU Int. 2012 Jun 6;111(1):159–170. doi: 10.1111/j.1464-410X.2012.11278.x

FIG. 4.

FIG. 4

Sildenafil (SIL) modulates the expression of glial cell line-derived neurotrophic factor receptor α2 (GFR-α2) in the pelvic ganglia after bilateral cavernosal nerve resection (BCNR). A, Protein extracts (as in Fig. 2) were subjected to western blot analysis for GFR-α2 and normalized by glyceraldehyde 3-phosphate dehydrogenase (GAPDH) with the correspondent densitometric analysis. B, Frozen pelvic ganglia sections from sham-operated (SHAM), BCNR and BCNR+SIL animals after 7 days were immunostained for GFR-α2 and subsequent detection by 3,3′-diaminobenzidine with no counterstaining. Representative pictures (magnification × 200; scale bar = 50 μm). Bottom: image analysis determined by the percentage of GFR-α per area and by the percentage total integrated optical density per cell. *P < 0.05 and **P < 0.01 with respect to Sham; #P < 0.05 with respect to BCNR.