TABLE 1.
Changes in the gene expression profile of neurotrophic-related pathways determined by quantitative reverse transcriptase-polymerase chain reaction (PCR) array 7 days after sham operation, bilateral cavernosal nerve resection (BCNR) and BCNR+sildenafil (BCNR+SIL) treatment
| Symbol | Description | Sham vsBCNR | BCNR vs BCNR+SIL |
|---|---|---|---|
| Artn | Artemin | −3.21 | 3.79 |
| Cntf | Ciliary neurotrophic factor | −2.38 | 2.17 |
| Gfra2 | GDNF family receptor α2 | −1.92 | 2.05 |
| nNOS | Neuronal nitric oxide synthase | −1.59 | 2.11 |
| Nrtn | Neurturin | −1.46 | 2.80 |
| Ntf-4 | Neurotrophin 4 | −2.48 | 2.4 |
Pooled major pelvic ganglia total RNA from sham-operated (Sham), BCNR and BCNR+SIL animals after 7 days was subjected to qRT-PCR array applying the Rat Neurotrophin and Receptors RT2 Profiler™ PCR Array. A list is provided of genes that are up- or down-regulated by BCNR in comparison with Sham and BCNR+SiL in comparison with BCNR. The ratios between the Sham vs BCNR and BCNR vs BCNR+SIL were normalized by ribosomal protein, large 1; ribosomal protein, large 3; and lactate dehydrogenase housekeeping genes, and were calculated for the assays performed in quadruplicate. Genes that were +1.8-fold up-regulated are shown underlined; genes down-regulated by −1.8-fold are shown in bold. Genes regulated by less than 1.8-fold are shown in normal text. Genes that show 1.8-fold changes or more are considered as significant.