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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Traffic. 2012 Oct 16;14(1):97–106. doi: 10.1111/tra.12014

Figure 5. G-Clathrin with Arf6(Q67L) has slowed FRAP recovery, consistent with longer coated membrane lifetime.

Figure 5

Cells were transfected with GFP-clathrin alone (red, circles) or co-transfected with GFP-clathrin and Arf6(Q67L) (blue, squares) or Arf1() (green, triangles). Following an initial continuous image stream (30 frames at 30 ms exposure), 50 pixel square regions were photobleached and recovery in G-clathrin monitored by capturing 1 sec streams (as above) every 5 s for the first 30s (see Methods for details). Inset: Recovery curves obtained over 180 sec, in which 1 sec streams were taken every 5 s for the first 30s, and then every 20s for the duration.