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. 1976 Jun;18(3):1088–1094. doi: 10.1128/jvi.18.3.1088-1094.1976

In vitro synthesis of A-particle structual protein by membrane-bound polyribosomes.

K K Lueders
PMCID: PMC354808  PMID: 178915

Abstract

On the basis of association with endoplasmic reticulum membranes, poyribosomes isolated from mouse myeloma MOPC-104E were separated into two classes, membrane bound and free. The membrane-bound and free polyribosomes were then compared for their capacity to incorporate [35S]methionine into A-particle proteins in vitro. As revealed by a radioimmunological assay method, labeling of A-particle protein occurred with the membrane-bound polyribosomes but not with the free polyribosomes. Peptide mapping of the immunoprecipitated, in vitro [35S]methionine-labeled product confirmed that A-particle protein had been synthesized in vitro.

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Selected References

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