Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1976 Jun;18(3):1088–1094. doi: 10.1128/jvi.18.3.1088-1094.1976

In vitro synthesis of A-particle structual protein by membrane-bound polyribosomes.

K K Lueders
PMCID: PMC354808  PMID: 178915

Abstract

On the basis of association with endoplasmic reticulum membranes, poyribosomes isolated from mouse myeloma MOPC-104E were separated into two classes, membrane bound and free. The membrane-bound and free polyribosomes were then compared for their capacity to incorporate [35S]methionine into A-particle proteins in vitro. As revealed by a radioimmunological assay method, labeling of A-particle protein occurred with the membrane-bound polyribosomes but not with the free polyribosomes. Peptide mapping of the immunoprecipitated, in vitro [35S]methionine-labeled product confirmed that A-particle protein had been synthesized in vitro.

Full text

PDF
1092

Images in this article

1093Image
on p.1093

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BERNHARD W. The detection and study of tumor viruses with the electron microscope. Cancer Res. 1960 Jun;20:712–727. [PubMed] [Google Scholar]
  2. Biczysko W., Pienkowski M., Solter D., Koprowski H. Virus particles in early mouse embryos. J Natl Cancer Inst. 1973 Sep;51(3):1041–1050. doi: 10.1093/jnci/51.3.1041. [DOI] [PubMed] [Google Scholar]
  3. Calarco P. G., Szollosi D. Intracisternal A particles in ova and preimplantation stages of the mouse. Nat New Biol. 1973 May 16;243(124):91–93. [PubMed] [Google Scholar]
  4. Chase D. G., Pikó L. Expression of A- and C-type particles in early mouse embryos. J Natl Cancer Inst. 1973 Dec;51(6):1971–1975. doi: 10.1093/jnci/51.6.1971. [DOI] [PubMed] [Google Scholar]
  5. DALTON A. J., POTTER M., MERWIN R. M. Some ultrastructural characteristics of a series of primary and transplanted plasma-cell tumors of the mouse. J Natl Cancer Inst. 1961 May;26:1221–1267. [PubMed] [Google Scholar]
  6. Gielkens A. L., Salden M. H., Bloemendal H. Virus-specific messenger RNA on free and membrane-bound polyribosomes from cells infected with Rauscher leukemia virus. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1093–1097. doi: 10.1073/pnas.71.4.1093. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kuff E. L., Leuders K. K., Ozer H. L., Wivel N. A. Some structural and antigenic properties of intracisternal A particles occurring in mouse tumors (complement fixation-immunodiffusion-neuroblastoma-plasma-cell tumor). Proc Natl Acad Sci U S A. 1972 Jan;69(1):218–222. doi: 10.1073/pnas.69.1.218. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lueders K. K., Kuff E. L. Synthesis and turnover of intracisternal A-particle structural protein in cultured neuroblastoma cells. J Biol Chem. 1975 Jul 10;250(13):5192–5199. [PubMed] [Google Scholar]
  9. Marciani D. J., Kuff E. L. Isolation and partial characterization of the internal structural proteins from murine intracisternal A particles. Biochemistry. 1973 Dec 4;12(25):5075–5083. doi: 10.1021/bi00749a008. [DOI] [PubMed] [Google Scholar]
  10. Marciani D. J., Kuff E. L. Structural proteins of intracisternal A-particles: possible repetitive sequences. J Virol. 1974 Dec;14(6):1597–1599. doi: 10.1128/jvi.14.6.1597-1599.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Moss B., Rosenblum E. N. Hydroxylapatite chromatography of protein-sodium dodecyl sulfate complexes. A new method for the separation of polypeptide subunits. J Biol Chem. 1972 Aug 25;247(16):5194–5198. [PubMed] [Google Scholar]
  12. Palacios R., Schimke R. T. Identification and isolation of ovalbumin-synthesizing polysomes. 3. Precipitation of ovalbumin polysomes from a heterologous cell-free protein-synthesizing system. J Biol Chem. 1973 Feb 25;248(4):1424–1430. [PubMed] [Google Scholar]
  13. Shafritz D. A. Protein synthesis with messenger ribonucleic acid fractions from membrane-bound and free liver polysomes. Translation characteristics of liver polysomal ribonucleic acids and evidence for albumin production in a messenger-dependent reticulocyte cell-free system. J Biol Chem. 1974 Jan 10;249(1):81–88. [PubMed] [Google Scholar]
  14. Vecchio G., Tsuchida N., Shanmugam G., Green M. Virus-specific messenger RNA and nascent polypeptides in polyribosomes of cells replicating murine sarcoma-leukemia viruses. Proc Natl Acad Sci U S A. 1973 Jul;70(7):2064–2068. doi: 10.1073/pnas.70.7.2064. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. WEBB T. E., BLOBEL G., POTTER V. R. POLYRIBOSOMES IN RAT TISSUES. I. A STUDY OF IN VIVO PATTERNS IN LIVER AND HEPATOMAS. Cancer Res. 1964 Aug;24:1229–1237. [PubMed] [Google Scholar]
  16. Wilson S. H., Bohn E. W., Matsukage A., Lueders K. K., Kuff E. L. Studies on the relationship between deoxyribonucleic acid polymerase activity and intracisternal A-type particles in mouse myeloma. Biochemistry. 1974 Mar 12;13(6):1087–1094. doi: 10.1021/bi00703a005. [DOI] [PubMed] [Google Scholar]
  17. Wivel N. A., Lueders K. K., Kuff E. L. Structural organization of murine intracisternal A particles. J Virol. 1973 Feb;11(2):329–334. doi: 10.1128/jvi.11.2.329-334.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Yang S. S., Wivel N. A. Analysis of high-molecular-weight ribonucleic acid associated with intracisternal A particles. J Virol. 1973 Feb;11(2):287–298. doi: 10.1128/jvi.11.2.287-298.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Zaanie D., Gielkens A. L., Dekker-michielsen M. J., Bloemers H. P. Virus-specific precursor polypeptides in cells infected with Rauscher leukemia virus. Virology. 1975 Oct;67(2):544–552. doi: 10.1016/0042-6822(75)90454-7. [DOI] [PubMed] [Google Scholar]
  20. de Harven E. Remarks on the ultrastructure of type A, B, and C virus particles. Adv Virus Res. 1974;19:221–264. doi: 10.1016/s0065-3527(08)60661-2. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES