Table 3.

The gradients used in the chromatographic system

Time (min)	Flow (mL/min)	%B
0.0	0.20	1.0
0.1		1.0
1.0	0.20
3.0		39.0
14.0	0.40	99.9
16.0	0.48	99.9
16.1		1.0
19.0	0.48
19.1	0.20

Linear gradients were used between set points