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RT-PCR for PSGCs and PLPCs during the 1 st passage under 2D and 3D cultivation conditions*
| Primer | 2D conditions | 3D conditions, 10thday | |
|---|---|---|---|
| PSGC | PLPC | PSGC | |
| mGAPDH | 1 | 1 | 1 |
| mKRT19 | 14.1 | 295.59 | 7.9 |
| mAFP | 0.01 | 0.12 | 1.19 |
| m1AAT | 0.04 | 0.30 | 3.19 |
| mTAT | 0.09 | 0.43 | 0.32 |
| mPEPCK | 0.02 | 0.08 | 0.18 |
* The data have been standardized with respect to GAPDH
