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. 2012 Dec 18;154(2):727–737. doi: 10.1210/en.2012-1774

Figure 1.

Figure 1.

Genistein protects thrombin-induced EC barrier dysfunction. A, BAEC monolayers were preincubated with various concentrations of genistein (G) or vehicle (C) for 30 minutes. Cells were then treated with or without 2U/ml thrombin (T) in the continued presence of genistein for 1 hour and avidin-FITC passage was measured. B, BAECs were preincubated with genistein (G, 5 μM) or vehicle (C) for 30 minutes, followed by stimulation with thrombin (T; 2 U/ml) for 5 minutes. Cells were then fixed with paraformaldehyde, permeabilized with Triton X-100, and stained with Alexa-phalloidin, and viewed with a digital fluorescence microscope. C, BAECs were preincubated with indicated concentrations of genistein for 30 minutes, followed by stimulation with thrombin (T; 2 U/ml) for 5 minutes. Phosphorylation of MLC (upper panel) and total MLC (lower panel) were detected by Western blot. Data are expressed as mean ± SE of four different experiments, each performed in duplicate. *P < .05 vs control; †P < .05 vs thrombin-alone-treated cells.