Figure 2.

Genistein does not affect thrombin-induced intracellular calcium release in BAECs. BAECs were loaded with 1 μg/mL of fura-2 AM (30 min at 37°C in culture medium). The cells were preincubated with various concentrations of genistein (G) or vehicle (C) for 30 minutes at 37°C and were then treated with or without 2 U/ml thrombin (T) for 5 minutes. [Ca²⁺] was measured in fura-2 loaded BAECs at an emission wavelength of 505 nm with alternating excitation wavelengths of 340 and 380 nm. Data are expressed as mean ± SE from three independent experiments, each assayed in duplicate. *P < .05 vs vehicle-treated control.