Table 5.
Femoral Histomorphometry of 3-month-old Male and Female conditional RosaNotch Mice and Controls
|
Osx-Cre;RosaNotch |
Dmp1-Cre;RosaNotch |
|||
|---|---|---|---|---|
| Control | Notch | Control | Notch | |
| Males | ||||
| Bone volume/tissue volume (%) | 10.5 ± 1.5 | 25.3 ± 2a | 6.3 ± 0.3 | 16.0 ± 3.0a |
| Trabecular separation (μm) | 292 ± 29 | 102 ± 7a | 403 ± 9 | 189 ± 50a |
| Trabecular number (1/mm) | 3.2 ± 0.3 | 7.4 ± 0.3a | 2.3 ± 0.1 | 5.2 ± 0.7a |
| Trabecular thickness (μm) | 32.3 ± 2.2 | 33.9 ± 1.4 | 27.0 ± 0.9 | 29.8 ± 2.1 |
| Osteoblast surface/bone surface (%) | 14.3 ± 1.8 | 43.4 ± 2.3a | 12.5 ± 1.9 | 18.8 ± 4.0 |
| Osteoblasts/bone perimeter (1/mm) | 13.3 ± 1.8 | 29.2 ± 1.9a | 13.3 ± 1.6 | 16.0 ± 3.0 |
| Osteoclast surface/bone surface (%) | 8.1 ± 0.3 | 7.5 ± 0.8 | 8.9 ± 0.5 | 12.4 ± 1.8b |
| Osteoclasts/bone perimeter (1/mm) | 5.1 ± 0.2 | 4.6 ± 0.6 | 6.2 ± 0.4 | 8.5 ± 1.1b |
| Eroded surface/bone surface (%) | 14.0 ± 0.6 | 15.2 ± 1.6 | 15.8 ± 0.8 | 23.9 ± 2.4a |
| Mineral apposition rate (μm/day) | Diffuse and fragmented labels | Diffuse and fragmented labels | ||
| Mineralizing surface/bone surface (%) | ||||
| Bone formation rate (μm3/μm2/d) | ||||
| Females | ||||
| Bone volume/tissue volume (%) | 3.8 ± 0.5 | 25.7 ± 1.3a | 4.2 ± 0.4 | 17.1 ± 4.6a |
| Trabecular separation (μm) | 621 ± 60 | 93 ± 4a | 582 ± 54 | 193 ± 53a |
| Trabecular number (1/mm) | 1.6 ± 0.1 | 8.0 ± 0.3a | 1.7 ± 0.1 | 5.1 ± 1.0a |
| Trabecular thickness (μm) | 23.5 ± 1.4 | 31.9 ± 0.9a | 24.5 ± 0.7 | 32.0 ± 4.2 |
| Osteoblast surface/bone surface (%) | 22.4 ± 1.8 | 45.1 ± 1.4a | 24.4 ± 1.0 | 26.9 ± 3.4 |
| Osteoblasts/bone perimeter (1/mm) | 24.0 ± 1.4 | 36.2 ± 0.9a | 26.1 ± 1.4 | 23.2 ± 2.7 |
| Osteoclast surface/bone surface (%) | 9.0 ± 0.5 | 6.8 ± 0.3a | 14.4 ± 0.8 | 13.1 ± 1.6 |
| Osteoclasts/bone perimeter (1/mm) | 6.0 ± 0.4 | 4.7 ± 0.3a | 9.3 ± 0.6 | 8.8 ± 1.2 |
| Eroded surface/bone surface (%) | 13.3 ± 0.8 | 13.4 ± 0.7 | 21.6 ± 1.6 | 22.7 ± 1.9 |
| Mineral apposition rate (μm/day) | 1.7 ± 0.1 | 1.4 ± 0.1 | Diffuse and fragmented labels | |
| Mineralizing surface/bone surface (%) | 4.8 ± 1.7 | 1.2 ± 0.2a | ||
| Bone formation rate (μm3/μm2/d) | 0.08 ± 0.03 | 0.02 ± 0.01a | ||
Bone histomorphometry was performed on femurs from 3 month old male and female Osterix (Osx)-Cre;RosaNotch and Dentin matrix protein1 (Dmp1)-Cre;RosaNotch and control littermates. Values are means ± sem; n = 4 to 7.
a
Significantly different from controls, P < 0.05 by unpaired t test;
b
P < 0.058.