Figure 6.

AZD8055 and Torin-1 show an inhibitory effect on survival of primary human pheochromocytoma cells. Dissociated cells from human tumor samples were plated at low density and treated with 1μM and 3μM AZD8055 (A) or 1μM Torin-1 (B) for 1 week. Control cells were incubated with DMSO for the same period of time. The cells were then fixed and stained for TH to discriminate tumor cells from other cell types in primary cultures. TH-positive cells were then counted to compare the number of treated cells with control cells. In the drug-containing culture, neoplastic chromaffin cells (red cytoplasm) are decreased in number and some are rounded (inset). Original magnification, ×20; expanded boxes show selected cells at higher magnification.