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. 2012 Dec 19;154(2):675–684. doi: 10.1210/en.2012-1818

Figure 2.

Figure 2.

Endogenous VNUT is critical for ATP release from pancreatic β cells. A, Measurement of ATP release from MIN6 cells stimulated with various concentrations of glucose (G) or KCl (50 mM). B, Representative confocal images showing MIN6 cells stained with quinacrine and FM4–64 under basal conditions (2.8 mM) and after a 10-minute challenge with high glucose (25 mM). C, Summary of quinacrine staining in MIN6 cells treated with 2.8 mM or 25 mM glucose. D, VNUT mRNA expression measured by real-time PCR from MIN6 cells infected with either control or shRNA VNUT lentiviral vectors for 72 hours. E, MIN6 cells were infected with control or shRNA VNUT lentiviral vectors 72 hours before being stained with quinacrine. Representative monolayers were imaged using confocal microscopy under basal conditions (2.8 mM). F, Summary of quinacrine staining in MIN6 cells infected with control or shRNA VNUT lentiviral vectors. G, ATP release from control or VNUT shRNA-infected MIN6 cells under basal conditions (2.8 mM glucose) or after 20 minutes of glucose stimulation (25 mM glucose). Scale bar: 20 μm. * P < .05, ** P < .01.