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. 2012 Dec 19;154(2):675–684. doi: 10.1210/en.2012-1818

Figure 3.

Figure 3.

VNUT-mediated ATP release is essential for insulin secretion. A, Insulin secretion from MIN6 cells infected with either control or VNUT shRNA lentiviral vectors under basal conditions (2.8 mM G), stimulated with glucose (16.8 mM G) or tolbutamide (200 μM) for 60 minutes. B, Insulin secretion from MIN6 cells pretreated with various concentrations of Evans blue for 30 minutes before stimulation with high glucose (16.8 mM G) for 60 minutes. C, VNUT mRNA expression measured by real-time PCR from isolated mouse islets infected with either control or shRNA VNUT lentiviral vector for 72 hours. D, Insulin secretion from isolated mouse islets infected with either control or VNUT shRNA lentiviral vectors under basal conditions (2.8 mM G), stimulated with glucose (16.8 mM G) or tolbutamide (200 μM) for 60 minutes. E, Insulin secretion from control and VNUT shRNA-infected islets stimulated with glucose (16.8 mM) in the presence or absence of exogenous ATP (200 μM) for 60 minutes. F, Insulin secretion from islets pretreated with various concentrations of Evans blue for 30 minutes before stimulation with glucose (16.8 mM) with or without exogenous ATP (200 μM) for 60 minutes. * P < .05, ** P < .01.