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. 2012 Dec 19;154(2):675–684. doi: 10.1210/en.2012-1818

Figure 4.

Figure 4.

Overexpression of VNUT enhances ATP release from β cells. A, VNUT mRNA expression measured by real-time PCR from MIN6 cells infected with either control or o/e VNUT lentiviral vectors for 72 hours. B, ATP release was measured from control and o/e VNUT MIN6 cells under basal conditions (2.8 mM glucose) or after 20 minutes of glucose stimulation (25 mM glucose). C, Representative confocal images showing quinacrine (green) and FM4–64 (red) staining in MIN6 cells infected with either control (upper panel) or o/e VNUT lentiviral vectors (lower panel) in basal media (2.8 mM glucose). D, Summary of quinacrine staining in control and o/e VNUT MIN6 cells. E, Quantification of the average number of quinacrine positive vesicles per 100 μm2. Scale bar: 20 μm. * P < .05, ** P < .01, *** P < .001.