Figure 5. Shear-induced actin cap formation is mediated by LINC complexes and nuclear lamin A/C.

(A) and (B). Percentages of Lmna^+/+ and Lmna^−/− MEFs featuring an organized actin cap (A) and organized basal stress fibers (B) in cells cultured in the absence/presence of shear flow of shear stresses of 0.05 dyn/cm² and 5 dyn/cm² applied for 5 min. Significances in main panels compare unsheared cells (black bars) to sheared cells (patterned bars). Inset graphs show same bars but compare differences between Lmna^+/+ and Lmna^−/− cells within the same shear condition. (C) and (D). Percentages of scramble control, nesprin2giant, and nesprin3 knockdown C2C12 cells featuring an organized actin cap (C) and organized basal stress fibers (D) in cells with the absence/presence of shear. Significances in main panels compare unsheared cells (black bars) to sheared cells (patterned bars) using two-way ANOVA tests. Inset graphs show same bars but compare differences between control and nesprin knockdown cells subjected to the same shear stress. For all data shown, ***, **,*, and ns indicate p value <0.001, <0.01, <0.05, and >0.05, respectively. α = 0.05 was used for all significance tests. Three independent experiments were conducted to quantify a total of 150 cells per condition.