Table 1.

Detection of TNF-α concentrations in dendrimer-modified plate, plate prepared by physical adsorption and commercial TNF-α kit using ELISA (TMB detection) method.

TNF-α Conc. (pg mL−1)	Dendrimer Plate (O.D.)	TNF-α Conc. (Cytok. mix.) (pg mL−1)	Dendrimer Plate (O.D.)	Dendrimer Plate Luminol (R.L.U.)	TNF-α Conc. (pg mL−1)	Physical Adsorption (O.D.)	TNF-α Kit (O.D.)
300	1.213	300	1.048	13414.6
240	1.067	240	0.800	10879.5	250	1.440	0.6625
80	0.493	80	0.423	4496.5	125	1.018	0.3335
26.6	0.270	26.6	0.262	1939.9	62.5	0.884	0.2045
8.88	0.192	8.88	0.188	1304.8	31.2	0.804	0.1070
2.96	0.151	2.96	0.171	1063.9	15.6	0.705	0.0655
0.98	0.137	0.98	0.164	876.8	7.5	0.646	0.0470
0.32	0.143	0.32	0.169	985.5	3.75	0.662	0.0420
0.11	0.141	0.11	0.158	975.5	1.87	0.618	0.0350
0.037	0.136	0.037	0.160	923.6	0.94	0.640	0.0335
0	0.138	0	0.164	834.1	0	0.576	0.0315
R2	0.996		0.995	0.995		0.980	0.9980
LLDa	0.15		0.17	943.0		0.68	0.0340
Sensitivity (pg mL−1)	<0.48		<1.14	<0.39		<8.0	<1.280

^aThe lower limit of detection (LLD) of TNF-α was determined by adding two STDs to the mean OD of three zero standard replicates and calculating the corresponding concentration from the standard curve.