Figure 3. Confocal fluorescence images of E. coli with the pH sensor.
A) Confocal fluorescence image of E. coli loaded with the sensor M. A size bar of 2 μm is included; B) Bright field image of E. coli loaded with M; C) Confocal fluorescence image of Bacillus sp. loaded with M. A size bar of 2 μm is included; D) Bright field image of Bacillus sp. loaded with M; E) Large area of fluorescence images of M in E. coli; F) Spectrometric analysis of the image shown in E. Spot 1 represents fluorescence from background. Spot 2, 3, 4 and 5 represent spectra from four selected areas in image 3E.