Figure 2.
ER/M accumulation of αS oligomers in human PD cases. A, Dot-blot analysis of BrSt microsome fractions from human control and PD cases. Microsomes were probed with antibodies against αS oligomers and pS129 αS. Quantitative data values are normalized for total αS. Values are given as the mean ± SEM (n = 8 for controls, n = 12 for PD). *p < 0.05, **p < 0.01, Student's t test. B, C, Total αS aggregates (TX-Ptot) and cytosolic fractions from PD brains do not show a significant accumulation of αS oligomers. TX-Ptot (B) or cytosolic (C) fractions from human PD cases were dot-blotted for FILA-1 and αS. The ER/M fractions are also shown in B.