FIGURE 5.

And-1 is required for the recruitment of new CENP-A to centromeres. A, cell cycle localization of And-1 and CENP-A. HCT116 cells were fixed and stained with anti-And-1 (red) and anti-CENP-A (green) antibodies and counterstained with DAPI (blue). B, schematic of experimental procedure performed in C. C, And-1 depletion impairing the centromeric localization of new CENP-A to centromeres. Left, HCT116 cells treated as described in B, preextracted and fixed for immunostaining for And-1 proteins. Right, quantification of the percentage cells with YFP-CENP-A centromeric signals in siGl2- and siAnd-1-treated HCT116 cells. Three independent experiments were performed, and at least 300 cells were counted for each condition. Data represent the means ± S.D. (error bars). Scale bars, 10 μm.