FIGURE 1.
The sequence and structure of wt EIAV-CA. A, the structure based sequence alignment between EIAV-CA (UniProt ID P69732) and HIV-1-CA (UniProt ID P12497). The CAN and CAC domains were separately aligned according to their three-dimensional structures (77). The crystal structure coordinates of EIAV-CA (PDB code 2EIA)(44), and HIV-1-CAN (PDB code 1AK4 or 1GWP)(23, 27) and HIV-1-CAC (PDB code 1A43)(78) were used. Identical and homologous residues were highlighted in red and green, respectively. The red arrows point to the conserved sequence affected by the β-hairpin formation identified in this study. B, the three-dimensional structure of EIAV-CA with the refined CAN domain possessing the β-hairpin. The cyan colored ribbons correspond to un-assigned residues with severe 1H-15N resonance line broadening.