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Autoproteolysis-dependent activation of TbSP1. Self-cleavage (empty dots) and PLA2 (filled dots) activities measured in parallel on samples of recombinant 22-kDa TbSP1 (120 μm) incubated for the indicated lengths of time at 37 °C. Maximum PLA2 activity after 16 h (with 10% residual undigested TbSP1) was 415 nmol of product/min/ng of protein. Error bars, S.D.
