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. 2012 Nov 28;288(3):1533–1547. doi: 10.1074/jbc.M112.384156

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FIGURE 9. — Temperature and pH dependence of the autoproteolytic and phospholipase activities of TbSP1. A, autoproteolytic activity (filled circles) of pro-TbSP1 (BL21-Ori, 20 μm), measured by SDS-PAGE after incubation for 15 h at the indicated temperatures in the presence of the PC substrate (5 mm), without calcium. Also shown is the phospholipase activity of pro-TbSP1 (filled squares) and mature TbSP1 (empty squares) (BL21-Ori, 0.05 μm), measured after a 10-min incubation at the indicated temperatures in the presence of 5 mm PC and 30 mm CaCl₂. B, pH dependence of TbSP1 autoproteolytic (22-kDa form; filled circles) and phospholipase (14.5-kDa form; empty squares) activities (see “Experimental Procedures” for details). Error bars, S.D.