FIGURE 4.
Effect of anti-serpin B13 natural autoantibodies on CD4 in the pancreas-associated lymphocytes. Left panels, analysis of CD4 expression in 4-week-old female NOD mice that had been prescreened for low (SBAlow) or high (SBAhigh) secretion of anti-serpin B13 autoantibodies and received either E64 or diluent (PBS) for 10 consecutive days exactly as described in the legend to Fig. 3. The animals were killed, and cell suspensions obtained from their organs were stained with PE-conjugated anti-CD4 mAb at 1:800. This dilution of anti-CD4 mAb allowed us to distinguish between high (M1) and low (M2) rates of expression of CD4 in T cells. Each histogram was generated by examining four animals. Right panels, the average of three experiments described is shown. The data are expressed as the ratio between populations with low versus high rates of CD4 expression. The errors bars indicate S.D. DMSO, dimethyl sulfoxide.