Skip to main content
. 2012 Nov 28;288(3):1620–1633. doi: 10.1074/jbc.M112.400929

FIGURE 8.

FIGURE 8.

Studies with triple knockdown cells. a, flow cytometry dot plot of WT and mutant HL-60 cells lacking between one to three α(1,3)FUTs. Expression of DsRed and cerulean confirm stable gene silencing. b–d, flow cytometry readings for CLA (b), sLeX (c), and LeX (d) epitopes. e, rolling and adherent cell density data for FUT4−7−9− and FUT7−9− cerulean HL-60 cells on L-E cell substrates at 1 dyne/cm2, compared with WT and FUT7−9− dual knockdowns. f and g, cell rolling and adhesion (f), and rolling velocity data (g) for various HL-60 cell lines interacting with IL-1β-stimulated HUVEC at 1 dyne/cm2. Data indicate a minor contribution of FUT4 to E-selectin mediated leukocyte rolling. *, p < 0.05 with respect to WT HL-60. †, p < 0.05 with respect to all other treatments, except that bars with daggers are not different from each other.