TABLE 1.

Yeast strains and plasmids used in this study

Strain	Genotype	Source or Ref.
FGY3	MATa, ura3-52, lys2-801, ade2-101, trp1-Δ1, his3-Δ200, leu2-Δ1	52
FGY2	MATa, ura 3-52, lys2-801, ade2-101, trp1-Δ1, his3-Δ200, leu2-Δ1, crd1Δ::URA3	52
FGY3 isu1Δ	MATa, ura 3-52, lys2-801, ade2-101, trp1-Δ1, his3-Δ200, leu2-Δ1, isu1Δ::KanMX4	This study
FGY2 isu1Δ	MATa, ura 3-52, lys2-801, ade2-101, trp1-Δ1, his3-Δ200, leu2-Δ1, crd1Δ::URA3, isu1Δ::KanMX4	This study
FGY3 isu2Δ	MATa, ura 3-52, lys2-801, ade2-101, trp1-Δ1, his3-Δ200, leu2-Δ1, isu2Δ::KanMX4	This study
FGY2 isu2Δ	MATa, ura 3-52, lys2-801, ade2-101, trp1-Δ1, his3-Δ200, leu2-Δ1, crd1Δ::URA3, isu2Δ::KanMX4	This study
pCM182	Low copy number plasmid, TRP1 nutritional marker	ATCC[regs] no. 87656
pCM182-ISU1	Derivative of pCM182, expresses ISU1 from the Tet-Off promoter	This study
pRS415	Low copy number plasmid, LEU2 nutritional marker	111
YEp351	High copy number plasmid, LEU2 nutritional marker	112
YEp351-YAP1	Derivative of YEp351, expresses YAP1 from the native promoter	42