FIGURE 2.
Effects of oxidizing and reducing reagents on GH expression. A, GH3 cells were cultured with 200 μm DTT, H2O2, or diamide in the presence of 10 nm T3 for 24 h, and mRNA revels of GH were determined by qRT-PCR. B, GH3 cells were cultured with 100 μm DTT or diamide in the presence of 10 nm T3. After 48 h, total cellular protein was isolated, and immunoblotting was performed. GH protein levels without T3 were set at 1.0. Values are expressed as the mean ± S.D. (error bars) for three replicates. **, p < 0.01; *, p < 0.05 compared with control. C, GH3 cells were treated with 0.1, 0.5, or 1 mm BSO in the presence or absence of 10 nm T3 for 24 h, and mRNA revels of GH were determined by qRT-PCR.